Abstract:

Objective To investigate the DNA repair gene RAD52 3'- untranslated region （3'- UTR） miRNA target sequence polymor- phisms and susceptibility to hepatocellular carcinoma（HCC） in Guangxi population. Methods A case- control study of 1，002 HCC cases and 1，013 cancer- free controls was performed.miRNA target sequence SNPs（rs1051669，rs1051672，rs7301931 and rs7310449） in the RAD52 gene 3'- UTR region were genotyped to analyze the relationship between the distribution frequency of SNP genotypes and genetic susceptibility to HCC. Results There was no significant difference in the distribution frequency of the SNP genotypes in the RAD52 between the case group and the controls（P>0.05）.After adjusting for age，sex，smoking，alcohol consumption and HBV infection，no significant association was found between the SNP genotypes and HCC susceptibility. Further stratification analysis showed that，compared with the rs1051669 C allele carrying genotypes，TT genotype can significantly reduce the risk of individuals suffering from HCC（TT vs CT/CC：OR=0.03，95%CI：0.00- 0.62，P=0.03），and compared with rs1051672 G alleles carrying genotypes，the AA genotype can significantly reduced the risk of HCC（AA vs GA/GG：OR=0.03，95%CI：0.01- 0.88，P=0.04） in the female. Conclusions RAD52 gene 3'- UTR region miRNA target sequence SNPs rs1051669 and rs1051672 might be associated with HCC susceptibility in female of Guangxi area.

Key words:  Liver neoplasms, RAD52, 3'- untranslated region, Single nucleotide polymorphism, Susceptibility