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中国癌症防治杂志

中国癌症防治杂志 ›› 2014, Vol. 6 ›› Issue (3): 224-229.doi: 10.3969/j.issn.1674-5671.2014.03.02

• 基础研究 • 上一篇    下一篇

肝细胞癌根治术后早期复发相关microRNAs的筛选

  

  1. 广西医科大学附属肿瘤医院肝胆外科；广西医科大学基础医学院免疫学教研室
  • 出版日期:2014-09-25 发布日期:2014-10-17
  • 通讯作者: 向邦德 E-mail:xiangbangde@163.com
  • 基金资助:

    国家科技重大专项课题（2012ZX10002010001009）；国家自然科学基金资助项目（81260331；81360312）；2014年广西研究生教育创新计划资助项目（YCSZ2014108）

Microarray-based screening to identify differentially expressed microRNAs associatedwith early recurrence of hepatocellular carcinoma after curative resection

  • Online:2014-09-25 Published:2014-10-17

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被引次数

2

摘要： 目的 筛选与肝细胞癌（hepatocellular carcinoma，HCC）患者根治术后早期复发相关的microRNAs。方法 收集10例HCC患者肝脏组织标本，其中早期复发组5例，非早期复发组5例，以及正常肝脏组织标本4例。利用microRNA基因芯片技术检测3组肝脏组织标本中microRNAs的表达谱，筛选出差异表达的microRNAs。结果 早期复发组与正常组相比，miR-21-3p、miR-21-5p、miR-222-3p、miR-3182、miR-3651、miR-3654、miR-4451、miR-4633-5p、miR-720的表达均上调，miR-4525的表达下调（P<0.05）；非早期复发组与正常组相比，miR-106b-5p、miR-4451、miR-3651、miR-93-5p的表达均上调，miR-451a、miR-3692-5p的表达下调（P<0.05）。结论 microRNAs的表达与HCC患者术后早期复发有关，检测患者肝脏组织中microRNAs的表达水平可能有助于监测HCC术后早期复发。

关键词: 肝肿瘤, microRNAs, 基因芯片, 复发

Abstract: Objective To screen for differentially expressed microRNAs associated with early recurrence of hepatocellular carcinoma （HCC）. Methods Tissue samples were taken from 10 HCC patients who had undergone curative resection， comprising 5 patients who experienced early recurrence and 5 who did not. In addition， normal tissue from 4 individuals without HCC served as control samples. All samples were analyzed using microarrays to identify microRNAs specifically expressed in early-recurrence HCC. Results Several microRNAs were significantly upregulated in early-recurrence HCC relative to normal tissue：miR-21-3p，miR-21-5p，miR-222-3p，miR-3182，miR-3651，miR-3654，miR-4451，miR-4633-5p，and miR-720. Conversely，miR-4525 was significantly downregulated in early-recurrence HCC relative to normal tissue. Relative to normal tissue，several microRNAs were significantly upregulated in non-early-recurrence HCC（miR-106b-5p，miR-3651，miR-4451，miR-93-5p） or downregulated（miR-3692-5p，miR-451a）. Conclusion MicroRNAs may play a key role in early recurrence of HCC after curative resection.

Key words: Liver neoplasm, MicroRNAs, Genechip, Recurrence