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中国癌症防治杂志

中国癌症防治杂志 ›› 2019, Vol. 11 ›› Issue (5): 387-392.doi: 10.3969/j.issn.1674-5671.2019.05.05

• 基础研究 • 上一篇    下一篇

基于ChinaPca全基因组数据和基因表达量探讨LRP1对前列腺癌发病的影响

  

  1. 广西医科大学附属肿瘤医院泌尿外科；广西医科大学研究生院；广西医科大学基因组与个体化医学研究中心；广西医科大学第一附属医院泌尿外科

  • 出版日期:2019-10-25 发布日期:2019-11-25
  • 通讯作者: 程继文 E-mail：chengjiwen1977@foxmail.com
  • 基金资助:
    国家自然科学基金项目（81660424）

Effect of LRP1 on the incidence of prostate cancer based on genome-wide ChinaPca data and gene expression

  • Online:2019-10-25 Published:2019-11-25

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摘要： 目的 探讨低密度脂蛋白受体相关蛋白-1（low density lipoprotein receptor-related protein-1，LRP1）基因对前列腺癌（prostate cancer，Pca）发病的影响。方法 在ChinaPca GWAS数据中对9个LRP1的单核苷酸多态性（single nucleotide polymorphism，SNP）进行前列腺癌易感性验证；取前列腺癌组织标本12例、癌旁正常组织18例和前列腺炎组织4例进行免疫组化检测；对TCGA和GTEx数据库的LRP1基因表达数据进行分析；在String网站构建LRP1基因的交互作用网络。结果 ChinaPca GWAS数据分析显示，LRP1基因的rs11172113位点可能是前列腺癌遗传易感位点（P=0.083），TC基因型与Pca发病风险有关（OR=1.267，95%CI：1.066~1.505，P=0.007）。免疫组化检测和TCGA数据库分析结果显示，LRP1在前列腺癌组织和癌旁正常组织中的表达量差异无统计学意义（P>0.05），整合TCGA和GTEx数据库数据后LRP1在前列腺癌组织中的表达降低（P＜0.05）。交互作用网络显示，LRP1与前列腺癌通路相关基因PLAT、PDGFB、PDGFRB有关联。结论 LRP1基因rs11172113位点可能是前列腺癌的遗传易感位点。LRP1基因在前列腺癌组织中呈低表达，可能通过调节PLAT、PDGFB和PDGFRB基因表达调控前列腺癌相关通路而导致前列腺癌发病。

关键词: 前列腺癌, 低密度蛋白受体相关蛋白1基因, 单核苷酸多态性, ChinaPca, 免疫组化

Abstract: Objective To investigate the effect of low density lipoprotein receptor-related protein-1 （LRP1） gene on prostate cancer （Pca）. Methods Based on ChinaPca GWAS data，nine single nucleotide polymorphism（SNP） of LRP1 were given to verify the prostate cancer susceptibility. Immunohistochemical tests were performed in 12 Pca tissues，18 normal prostate tissues and 4 prostatitis tissues，respectively. LRP1 gene expression data from TCGA and GTEx databases were analyzed，and the interaction network of LRP1 genes on the String website was built. Results ChinaPca GWAS data showed that rs11172113 of LRP1 gene might be the susceptible site of Pca（P=0.083），and TC genotype was related to the risk of Pca（OR=1.267，95%CI：1.066-1.505，P=0.007）. The results of immuno - histochemistry experiment and TCGA database analysis showed that there was no statistically significant difference in the expression of LRP1 in Pca tissues and normal prostate tissues（P>0.05）. After integrating the data in TCGA and GTEx databases，the expression of LRP1 in Pca tissues was decreased（P<0.05）. The interaction network showed that LRP1 was associated with the PLAT，PDGFB and PDGFRB  genes  which related to the prostate cancer pathway. Conclusions rs11172113 of LRP1 may be a genetic predisposition to prostate cancer. The low expression of LRP1 gene in prostate cancer. LRP1 may regulate the prostate cancer-related pathways by regulating the expression of PLAT，PDGFB and PDGFRB genes and affect the incidence of prostate cancer.

Key words: Prostate cancer, Low-density lipoprotein receptor-related protein 1, Single nucleotide polymorphism, ChinaPca, Immuno-histochemical

中图分类号: 

  • R737.25