Abstract:

Objective To study the relationship between single nucleotide polymorphism （SNP） rs2298141 in the integrin beta1 gene （Itgb1） and genetic susceptibility to hepatocellular carcinoma（HCC） in clusters of families with liver cancer in Fusui County，Guangxi. Methods In this case-control study，20 liver cancer family groups comprising 79 members served as the case，and 10 normal family groups comprising 40 members served as the control. Time-of-flight mass spectrometry was used to detect rs2298141 genotype frequencies，and logistic regression was carried out to explore correlations between these frequencies and HCC risk. Results The frequencies of the genotypes AA，AG and GG in controls was 40.00%，47.50% and 12.50%，compared to 50.00%，40.00% and 10.00% in the liver family group. Allele distributions did not differ significantly between two groups（P>0.05），and genotype distributions conformed to Hardy-Weinberg equilibrium. Among controls，risk of HCC in individuals with the AG genotype was 0.67（95%CI：0.22-2.1，P=0.50） relative to the risk in individuals with AA genotype；risk of HCC in those with the GG genotype was 1.05 （95%CI：0.17-6.6，P=0.96） relative to the risk in those with the AA genotype. The corresponding risk in families with high HCC incidence was 0.91（95%CI：0.31-2.71，P=0.86） and 2.2（95%CI：0.40-11.96，P=0.37）. Conclusion The Itgb1 rs2298141 polymorphism may not correlate with liver cancer in familial clusters of genetic susceptibility in Fusui County，Guangxi.

Key words: Liver neoplasm, Integrin-beta1 gene, Family, Single gene nucleotide polymorphism, Genetic susceptibility