Abstract: Objective To study the dynamic expression of resistance genes during chemotherapy resistance process，so as to provide evidences for exploring reversal targets and reversal treatment. Methods Adopt Benjamini-Hochberg（BH）to analyse expression profile data of ovarian cancer who provided by TCGA，then use KEGG of DAVID to enrich the pathway，then employ T test to measure them again，use COREMINE to select some genes which is associated with multiple drug resistant and drug resistance of neoplasm.Take the differentially expressed gene to conduct survival analysis，find out the genes which is significant. Then employ qRT-PCR to examine the expression of them in SKOV3-GFP and SKOV3/DDPⅱtumor. Result 306 differentially expressed genes were found in Microarray，of which 110 had increased expression and 196 had low  expression. KEGG of DAVID enrichment 5 up-regulate-pathway and 4 down-regulate-pathway.T test sift out 18 up-regulate genes and 19 down-regulate genes.COREMINE search out 5 up-regulate genes，for example：ITPA、IMPDH2、RPS7、PDE5A and PDE4D，down-regurate genes as GNAS、CFTR、BUB3、PRKDC、RBL2、SMC1A、CALR、CCNE2 and CHEK1. Survival analysis shows that overall survival was longer when comparing high expression team of CALR and PRKDC with low expression team.Expression of CALR and PRKDC gradually reduces as Cisplatin injection times increase in SKOV3-GFP and SKOV3/DDPⅱtumor. Conclusion Expression of CALR and PRKDC may be associated  with cisplatin resistance.So reducing expression of CALR and PRKDC mean that these ovarian cancer patientsmay have resistance to cisplatin.

Key words: Ovarian neoplasms, Epithelial ovarian cancer, Resistance genes, Cisplatin, Genetic expression, Dynamic change