Abstract: Objective To screen chromosomal aberration in hepatocellular carcinoma (HCC) and to explore the clinical signification.  Methods  Chromosomal aberration was detected by comparative genomic hybridization (CGH) in 25 patients with HCC，and the correlation between chromosomal aberration and clinical parameters were analyzed. Results All patients presented certain aberrations in copy number of chromosomal DNA. The amplification of copy number was commonly detected in chromosome 1q (72.0%)，1p (64.0%)，2q (48.0%)，2q (48.0%)，5q (48.0%) and 7q (44.0%)，while the loss of copy number was often found in chromosome 4q (48.0%)，16p (48.0%)，8p (40.0%) and 17p (36.0%).  The amplification of 17p and 18p as well as the loss of 8p，13q，11q and 8q showed significant correlation with some clinical parameters. Conclusions Chromosomal aberrations occur frequently in HCC. Some of the nonrandom chromosomal alterations may relate to the development，biological behavior and prognosis of HCC.

Key words: Liver neoplasm, Hepatocellular carcinoma (HCC), Comparative genomic hybridization (CGH), Chromosomal aberration, Prognosis of HCC