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25 September 2009, Volume 1 Issue 03 Previous Issue    Next Issue

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Effect of placental immunoregulatory factor detected by SELDI-TOF-MS on protein profiling of CIK-cell MENG Yi1,YANG Tie-zheng2,ZHANG Xue-rong3,et al. 2009, 1 (03):  193-196.  Abstract ( 100 )   Save Objective SELDI technology was used to detect the changes of protein expression in cytokine-induced killer cells(CIK) which were culture in the medium contains placental factor(PF).Methods Peripheral blood mononuclear cells(PBMNCs) were collected from healthy human donors.After being induced as cytokine induced-killer(CIK),the cells were divided into two groups.The CIK group was cultured with 10% FBS culture media only,and the PF-CIK group was cultured with 10% FBS culture media that was added placental factor(PF).The proteomic spectra of CIK were obtained by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS) on WCX-2 chips.The differences of proteins between CIK group and PF-CIK group were compared.Results The results of SELDI showed that a total of 6 protein was significantly different(P<0.05),especially the four with molecular weight as 5636.077,6860.359,6072.814 and 5984.191.Conclusions PF might have the function that can change the expression level of CIK.SELDI technology can find out the differentially expressed protein in CIK effectively. References | Related Articles | Metrics

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Effect of placental immunoregulatory factor detected by SELDI-TOF-MS on protein profiling of CIK-cell MENG Yi1,YANG Tie-zheng2,ZHANG Xue-rong3,et al. 2009, 1 (03):  193-196.  Abstract ( 40 )   Save Objective SELDI technology was used to detect the changes of protein expression in cytokine-induced killer cells(CIK) which were culture in the medium contains placental factor(PF).Methods Peripheral blood mononuclear cells(PBMNCs) were collected from healthy human donors.After being induced as cytokine induced-killer(CIK),the cells were divided into two groups.The CIK group was cultured with 10% FBS culture media only,and the PF-CIK group was cultured with 10% FBS culture media that was added placental factor(PF).The proteomic spectra of CIK were obtained by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS) on WCX-2 chips.The differences of proteins between CIK group and PF-CIK group were compared.Results The results of SELDI showed that a total of 6 protein was significantly different(P<0.05),especially the four with molecular weight as 5636.077,6860.359,6072.814 and 5984.191.Conclusions PF might have the function that can change the expression level of CIK.SELDI technology can find out the differentially expressed protein in CIK effectively. References | Related Articles | Metrics

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Effect of nitric oxide on growth of nasopharyngeal carcinoma cell lines and expression of vascular endothelial growth factor YI Xiang1,TANG An-zhou1,QIN Ying2,et al. 2009, 1 (03):  197-199.  Abstract ( 117 )   Save Objections To study the effective of nitric oxide(NO) on growth of nasopharyngeal carcinoma(NPC) cell lines,and on modulation of VEGF expression.Methods NPC cell lines CNE2 and TWO3 were treated with NO donor S-nitroso-N-acetylpenicillamine(SNAP).NO concentrations in cell culture medium were detected by nitrate reductase method.The survival rates of NPC cell lines were detected by MTT method.The expression of VEGF was detected by reverse transcription(RT-PCR) and western blot.Results NO inhibited growth of NPC cell lines in higher concentrations,while the low concentration of NO had no significant effect on NPC cell lines survival rate.NO modulated the expression of VEGF in NPC cell lines.Conclusions NO as a signal molecule plays a role in modulating the expression of VEGF,therefore it contributes to the development of NPC.Meanwhile,the high concentrations of NO can inhibit the development of NPC. References | Related Articles | Metrics

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Effect of nitric oxide on growth of nasopharyngeal carcinoma cell lines and expression of vascular endothelial growth factor YI Xiang1,TANG An-zhou1,QIN Ying2,et al. 2009, 1 (03):  197-199.  Abstract ( 50 )   Save Objections To study the effective of nitric oxide(NO) on growth of nasopharyngeal carcinoma(NPC) cell lines,and on modulation of VEGF expression.Methods NPC cell lines CNE2 and TWO3 were treated with NO donor S-nitroso-N-acetylpenicillamine(SNAP).NO concentrations in cell culture medium were detected by nitrate reductase method.The survival rates of NPC cell lines were detected by MTT method.The expression of VEGF was detected by reverse transcription(RT-PCR) and western blot.Results NO inhibited growth of NPC cell lines in higher concentrations,while the low concentration of NO had no significant effect on NPC cell lines survival rate.NO modulated the expression of VEGF in NPC cell lines.Conclusions NO as a signal molecule plays a role in modulating the expression of VEGF,therefore it contributes to the development of NPC.Meanwhile,the high concentrations of NO can inhibit the development of NPC. References | Related Articles | Metrics

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Correlation of hypoxia inducible factor 1-α polymorphism and nasopharyngeal carcinoma ZHANG Yong,YU Bin 2009, 1 (03):  200-202.  Abstract ( 134 )   Save Objective To investigate the distribution of hypoxia inducible factor 1α(HIF-1α) C2028T polymorphism in patients with nasopharyngeal carcinoma(NPC) and its role in NPC carcinogenesis.Methods DNA genome from 90 patients with NPC were extracted,and the HIF-1α C2028T polymorphism was detected by PCR-RFLP method.The relations of HIF-1α C2028T polymorphism with the patients' clinicopathologic features were statically analyzed.Results There were only two genotypes,C/C and C/T.The proportions of genotype C/C and C/T were 87.8%(79/90)and 12.2%(11/90)respectively.The HIF-1α C2028T polymorphism was positively related to patients' N stage(rs=0.39,P=0.00),and was likely having a positive correlation with UICC stage(rs=0.21,P=0.05).There was no correlation between the polymorphism and pathological type(P=0.98) or T stage(P=0.88).Conclusions The genotype C/T of HIF-1α might improve the progress of NPC carcinogenesis,especially might improve the metastasis of NPC.Its exact mechanism deserves further study. References | Related Articles | Metrics

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Correlation of hypoxia inducible factor 1-α polymorphism and nasopharyngeal carcinoma ZHANG Yong,YU Bin 2009, 1 (03):  200-202.  Abstract ( 71 )   Save Objective To investigate the distribution of hypoxia inducible factor 1α(HIF-1α) C2028T polymorphism in patients with nasopharyngeal carcinoma(NPC) and its role in NPC carcinogenesis.Methods DNA genome from 90 patients with NPC were extracted,and the HIF-1α C2028T polymorphism was detected by PCR-RFLP method.The relations of HIF-1α C2028T polymorphism with the patients' clinicopathologic features were statically analyzed.Results There were only two genotypes,C/C and C/T.The proportions of genotype C/C and C/T were 87.8%(79/90)and 12.2%(11/90)respectively.The HIF-1α C2028T polymorphism was positively related to patients' N stage(rs=0.39,P=0.00),and was likely having a positive correlation with UICC stage(rs=0.21,P=0.05).There was no correlation between the polymorphism and pathological type(P=0.98) or T stage(P=0.88).Conclusions The genotype C/T of HIF-1α might improve the progress of NPC carcinogenesis,especially might improve the metastasis of NPC.Its exact mechanism deserves further study. References | Related Articles | Metrics

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The effect of serum cardiac troponin Ⅰ level and myocardial ultrastructure on peri-anesthesia rats after Adriamycin chemotherapy HE Bing-wen,YANG Peng-ju,HUANG Bing,et al. 2009, 1 (03):  203-207.  Abstract ( 102 )   Save Objective To investigate the degree and characteristic of myocardial injury on peri-anesthesia wistar rats induced by Adriamycin(ADM) chemotherapy,through analysing the changes of serum cardiac troponin I(cTnI) level,creatine kinase(CK),isoenzyme of creatine kinase(CKMB) activites and myocardial ultrastructure.Methods Thirty-two wistar rats were randomized into four groups:A,B,C and D.Rats in group A and B were treated with ADM by intraperitoneal injection(ip),and rats in group C and D were treated with sodium chloride solution.All rats were treated with anesthesia on the third day after completing the last injection.Anesthesia treatment for the rats in group A and C were opened isoflurane inhalation,while for the rats in group B and D were sodium chloride pentobarbital solution by ip.Blood samples from all animal were taken at pre-anesthesia and 20min post-anaesthesia respectively.The hisptopathological changes of myocardium were observed with light microscope and transmission electron microscope.Results ①The differences of serum cTnI level among the four groups were not statistically significant at pre-anesthesia,but were statistically significant between group A and B,group B and D at post-anesthesia(P<0.01).The cTnI levels in all of the four groups were higher at post-anesthesia than that at pre-anesthesia,with the statistical significance in Group B(P<0.05).②The levels of serum CK and CKMB between pre-anesthesia and post-anesthesia were not statistically significant in four groups(P>0.05).③ With light and electron transmission microscope,pathological changes of myocardium were significant between chemotherapy groups and control groups,as the severe cell degeneration and necrosis were observed in chemotherapy group.The histopathological score of myocardium was significantly different among the four groups.Conclusions Serum cTnI level is a sensitive marker of cardiac injury at peri-anesthesia,and it is more sensitive and specific than CK and CKMB levels.Serum cTnI level also is an important marker to evaluate aggravation of cardiac injury induced by anesthesia on ADM chemotherapy.Inhalation isoflurane have no significant effect on aggravating cardiac injury induced by ADM chemotherapy comparied with sodium chloride pentobarbital,but provides certain degree of myocardial-protection effect. References | Related Articles | Metrics

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The effect of serum cardiac troponin Ⅰ level and myocardial ultrastructure on peri-anesthesia rats after Adriamycin chemotherapy HE Bing-wen,YANG Peng-ju,HUANG Bing,et al. 2009, 1 (03):  203-207.  Abstract ( 57 )   Save Objective To investigate the degree and characteristic of myocardial injury on peri-anesthesia wistar rats induced by Adriamycin(ADM) chemotherapy,through analysing the changes of serum cardiac troponin I(cTnI) level,creatine kinase(CK),isoenzyme of creatine kinase(CKMB) activites and myocardial ultrastructure.Methods Thirty-two wistar rats were randomized into four groups:A,B,C and D.Rats in group A and B were treated with ADM by intraperitoneal injection(ip),and rats in group C and D were treated with sodium chloride solution.All rats were treated with anesthesia on the third day after completing the last injection.Anesthesia treatment for the rats in group A and C were opened isoflurane inhalation,while for the rats in group B and D were sodium chloride pentobarbital solution by ip.Blood samples from all animal were taken at pre-anesthesia and 20min post-anaesthesia respectively.The hisptopathological changes of myocardium were observed with light microscope and transmission electron microscope.Results ①The differences of serum cTnI level among the four groups were not statistically significant at pre-anesthesia,but were statistically significant between group A and B,group B and D at post-anesthesia(P<0.01).The cTnI levels in all of the four groups were higher at post-anesthesia than that at pre-anesthesia,with the statistical significance in Group B(P<0.05).②The levels of serum CK and CKMB between pre-anesthesia and post-anesthesia were not statistically significant in four groups(P>0.05).③ With light and electron transmission microscope,pathological changes of myocardium were significant between chemotherapy groups and control groups,as the severe cell degeneration and necrosis were observed in chemotherapy group.The histopathological score of myocardium was significantly different among the four groups.Conclusions Serum cTnI level is a sensitive marker of cardiac injury at peri-anesthesia,and it is more sensitive and specific than CK and CKMB levels.Serum cTnI level also is an important marker to evaluate aggravation of cardiac injury induced by anesthesia on ADM chemotherapy.Inhalation isoflurane have no significant effect on aggravating cardiac injury induced by ADM chemotherapy comparied with sodium chloride pentobarbital,but provides certain degree of myocardial-protection effect. References | Related Articles | Metrics

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Expression and significance of MMP-11 in early breast cancer QIN Yu-zhou1,OU Hai-ling2,LIU Jian-lun1. 2009, 1 (03):  208-210.  Abstract ( 114 )   Save Objective To observe retrospectively the protein expression and significance of MMP11 in early breast cancer(EBC),and its relationship with clinical pathological features and the expression conditions of ER,PR and HER2.Methods A total of 76 cases with EBC were followed up in this study.The protein expressions of MMP11,ER,PR and HER2 in tumors were detected by immunohistochemical staining and statistical analysis.Results The positive expression rate of MMP11 protein was 68.42%.The expression of MMP11 was not related to the features such as histopathological type,PR expression,age and prognosis of the patients(P>0.05),but positive related to menopausal status of patient and ER expression of tumor(P<0.05).Univariate analysis showed the expressions of MMP11,ER and HER2 were correlated with prognosis,but multivariate analysis showed none of these protein expression related to prognosis.Conclusions The results of this small pilot study suggest that the expression of MMP11 protein in EBC was correlated with menopausal status and ER expression,but not correlated with prognosis.The role of MMP11 in EBC requires further study. References | Related Articles | Metrics

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Expression and significance of MMP-11 in early breast cancer QIN Yu-zhou1,OU Hai-ling2,LIU Jian-lun1. 2009, 1 (03):  208-210.  Abstract ( 75 )   Save Objective To observe retrospectively the protein expression and significance of MMP11 in early breast cancer(EBC),and its relationship with clinical pathological features and the expression conditions of ER,PR and HER2.Methods A total of 76 cases with EBC were followed up in this study.The protein expressions of MMP11,ER,PR and HER2 in tumors were detected by immunohistochemical staining and statistical analysis.Results The positive expression rate of MMP11 protein was 68.42%.The expression of MMP11 was not related to the features such as histopathological type,PR expression,age and prognosis of the patients(P>0.05),but positive related to menopausal status of patient and ER expression of tumor(P<0.05).Univariate analysis showed the expressions of MMP11,ER and HER2 were correlated with prognosis,but multivariate analysis showed none of these protein expression related to prognosis.Conclusions The results of this small pilot study suggest that the expression of MMP11 protein in EBC was correlated with menopausal status and ER expression,but not correlated with prognosis.The role of MMP11 in EBC requires further study. References | Related Articles | Metrics

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Applying EBV Rta-IgG in diagnosis of nasopharyngeal carcinoma ZHU Wen-liang,LIANG Xin-qiang,ZHANG Yang,et al. 2009, 1 (03):  211-213.  Abstract ( 230 )   Save Objective To detect the expression level of Rta-IgG in the untreated patients with nasopharyngeal carcinoma(NPC).Methods The diagnostic kit for IgG antibody to EBV Rta(ELISA) was used to test serum samples derived from 274 patients that were within different clinical stages confirmed by pathology examination,and 353 healthy controls.Results Significant difference of Rta-IgG positive rate between NPC group and healthy control group was revealed(P<0.01).However,the expression levels of Rta-IgG in NPC patients' serum were irrelative with the clinical stage of the tumor.The A value was significantly higher in NPC group than that in healthy control group(P<0.01).Conclusion Rta-IgG is a sensitive and specific serologic parameter for NPC diagnosis,but it is not a proper marker for evaluating the clinical stage of NPC. References | Related Articles | Metrics

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Applying EBV Rta-IgG in diagnosis of nasopharyngeal carcinoma ZHU Wen-liang,LIANG Xin-qiang,ZHANG Yang,et al. 2009, 1 (03):  211-213.  Abstract ( 69 )   Save Objective To detect the expression level of Rta-IgG in the untreated patients with nasopharyngeal carcinoma(NPC).Methods The diagnostic kit for IgG antibody to EBV Rta(ELISA) was used to test serum samples derived from 274 patients that were within different clinical stages confirmed by pathology examination,and 353 healthy controls.Results Significant difference of Rta-IgG positive rate between NPC group and healthy control group was revealed(P<0.01).However,the expression levels of Rta-IgG in NPC patients' serum were irrelative with the clinical stage of the tumor.The A value was significantly higher in NPC group than that in healthy control group(P<0.01).Conclusion Rta-IgG is a sensitive and specific serologic parameter for NPC diagnosis,but it is not a proper marker for evaluating the clinical stage of NPC. References | Related Articles | Metrics

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The relationship of Helicobacter pylori infection and the expression of alpha-catenin in gastric cancer HUANG Zhen-ning,QIN Shan-yu,JIANG Hai-xing,et al. 2009, 1 (03):  214-217.  Abstract ( 126 )   Save Objective To investigate the expression and the clinical significance of Alpha-Catenin(α-Cat) in carcinogenesis of gastric mucosa and its relationship with Helicobacter pylori(Hp) infection.Methods SP immunohistochemical technique was used to detect the expression of α-Cat in 96 cases of gastric carcinoma tissue and their adjacent tissues,and 15 control tissues.Rapid urease test and histological staining Warthin-Starry were used to determine the status of Hp infection.Results The positive rates of α-Cat expression in gastric cancer tissues,cancer-adjacent tissues and control tissues were 34.4%,56.2% and 100% respective1y(P<0.05).The expression of α-Cat in the Hp-positive cancer tissues was markedly lower than that in Hp-negative ones(36.2% vs.51.8%,P<0.05).The positive rate of α-Cat in cancer tissue was positively paralleled with the increase of differentiation grade,but there was no significant difference between the positive rate of α-Cat and the depth of tumor invasion or lymph node metastasis(P>0.05).Conclusions The expression of α-Cat was gradually down-regulated during the course of gastric carcinogenesis.Hp infection is closely related to the expression of α-Cat in gastric cancer.Hp infection might be an initiating factor,and the activation of α-Cat might be one of the key events in the pathogenesis of gastric cancer.Hp infection and α-Cat expression play synergic roles in occurrence and development of gastric cancer. References | Related Articles | Metrics

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The relationship of Helicobacter pylori infection and the expression of alpha-catenin in gastric cancer HUANG Zhen-ning,QIN Shan-yu,JIANG Hai-xing,et al. 2009, 1 (03):  214-217.  Abstract ( 62 )   Save Objective To investigate the expression and the clinical significance of Alpha-Catenin(α-Cat) in carcinogenesis of gastric mucosa and its relationship with Helicobacter pylori(Hp) infection.Methods SP immunohistochemical technique was used to detect the expression of α-Cat in 96 cases of gastric carcinoma tissue and their adjacent tissues,and 15 control tissues.Rapid urease test and histological staining Warthin-Starry were used to determine the status of Hp infection.Results The positive rates of α-Cat expression in gastric cancer tissues,cancer-adjacent tissues and control tissues were 34.4%,56.2% and 100% respective1y(P<0.05).The expression of α-Cat in the Hp-positive cancer tissues was markedly lower than that in Hp-negative ones(36.2% vs.51.8%,P<0.05).The positive rate of α-Cat in cancer tissue was positively paralleled with the increase of differentiation grade,but there was no significant difference between the positive rate of α-Cat and the depth of tumor invasion or lymph node metastasis(P>0.05).Conclusions The expression of α-Cat was gradually down-regulated during the course of gastric carcinogenesis.Hp infection is closely related to the expression of α-Cat in gastric cancer.Hp infection might be an initiating factor,and the activation of α-Cat might be one of the key events in the pathogenesis of gastric cancer.Hp infection and α-Cat expression play synergic roles in occurrence and development of gastric cancer. References | Related Articles | Metrics

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Clinical review of transcatheter hepatic arterial chemoembolization combined with microwave ablation in patients with advanced liver cancer ZHAO Chang,MA Yi-long,KANG Ping,et al. 2009, 1 (03):  218-220.  Abstract ( 210 )   Save Objective To evaluate the clinical effect and application value of transcatheter hepatic arterial chemoembolization(TACE) combined with percutaneous microwave ablation therapy(PMCT) in patients with advanced liver cancer.Methods Eighty-three patients with stage Ⅱa-Ⅲa liver cancer were divided into two groups.Twenty-eight patients undertook the therapy that combined TACE and PMCT as the therapy group.Fifty-five patients performed only with TACE were enrolled in control group.Patients were treated with TACE first.CT inspection or DSA was performed every 3-4 weeks after treatment.TACE or TACE+PMCT were selected again according to the condition of fistula and tumor.Three continuous treatments was a treatment period.Then the therapeutic effect rates,side effects,rates of recurrence and metastasis,survival time of the two groups were followed up.Results The effect rates were 67.86% and 40.00% in the therapy group and control group respectively(P<0.05).The median survival time,and the 0.5-,1-,1.5-,2-year cumulative survival rate were 30 months,100%,93%,79% and 61% in therapy group respectively,while were 24months,95%,84%,65% and 36% in control group respectively(P<0.05).The 0.5-,1-and 2-year cumulative recurrence and metastasis rate were 7.14%,14.29% and 32.14% in therapy group respectively,while were 9.09%,34.55% and 65.45% in control group respectively(P<0.05).All of patients in the two groups had no severe complications.Eight HAPS in the control group were blocked up with TACE,three of them were recanalized later.Five HAPS in the therapy group were completely blocked,but none of them was recanalized.Conclusions TACE combined with PMCT can safely and effectively treat the patients with advanced liver cancer,and can extend life span,reduce the recurrence rate without increasing side effects. References | Related Articles | Metrics

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Clinical review of transcatheter hepatic arterial chemoembolization combined with microwave ablation in patients with advanced liver cancer ZHAO Chang,MA Yi-long,KANG Ping,et al. 2009, 1 (03):  218-220.  Abstract ( 78 )   Save Objective To evaluate the clinical effect and application value of transcatheter hepatic arterial chemoembolization(TACE) combined with percutaneous microwave ablation therapy(PMCT) in patients with advanced liver cancer.Methods Eighty-three patients with stage Ⅱa-Ⅲa liver cancer were divided into two groups.Twenty-eight patients undertook the therapy that combined TACE and PMCT as the therapy group.Fifty-five patients performed only with TACE were enrolled in control group.Patients were treated with TACE first.CT inspection or DSA was performed every 3-4 weeks after treatment.TACE or TACE+PMCT were selected again according to the condition of fistula and tumor.Three continuous treatments was a treatment period.Then the therapeutic effect rates,side effects,rates of recurrence and metastasis,survival time of the two groups were followed up.Results The effect rates were 67.86% and 40.00% in the therapy group and control group respectively(P<0.05).The median survival time,and the 0.5-,1-,1.5-,2-year cumulative survival rate were 30 months,100%,93%,79% and 61% in therapy group respectively,while were 24months,95%,84%,65% and 36% in control group respectively(P<0.05).The 0.5-,1-and 2-year cumulative recurrence and metastasis rate were 7.14%,14.29% and 32.14% in therapy group respectively,while were 9.09%,34.55% and 65.45% in control group respectively(P<0.05).All of patients in the two groups had no severe complications.Eight HAPS in the control group were blocked up with TACE,three of them were recanalized later.Five HAPS in the therapy group were completely blocked,but none of them was recanalized.Conclusions TACE combined with PMCT can safely and effectively treat the patients with advanced liver cancer,and can extend life span,reduce the recurrence rate without increasing side effects. References | Related Articles | Metrics

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Relationship between expression of VEGF and recurrence of Hepatocellular Carcinoma 1MO Xian-wei,2LIN Yuan 2009, 1 (03):  221-222.  Abstract ( 176 )   Save Objective To explore the relationship between expression of vascular endothelial growth factor(VEGF) and recurrence of hepatocellular carcinoma(HCC).Methods 71 cases of patients with HCC were grouped into VEGF-positive expression(VP) and VEGF-negative expression(VN).Parameters from the two groups such as classification of cancer cells,tumor capsule,vein tumor thrombus,recurrence rate in 1 year and recurrence rate between 1 and 3 years were compared.Results There were no significant differences between the two groups at classification of cancer cells and recurrence rate between 1 and 3 years(P>0.05).The rate of vein tumor thrombus and recurrence rate in 1 year were higher in group VP than that in group VN(P<0.05).The rate of tumor capsule was lower in group VP than that in group VN(P<0.05).Conclusions Expression of VEGF is correlated with early recurrence of HCC,possibly through the role of micro-metastasis by cancer cells.Expression of VEGF is not significantly correlated with the late recurrence of HCC. References | Related Articles | Metrics

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Relationship between expression of VEGF and recurrence of Hepatocellular Carcinoma 1MO Xian-wei,2LIN Yuan 2009, 1 (03):  221-222.  Abstract ( 90 )   Save Objective To explore the relationship between expression of vascular endothelial growth factor(VEGF) and recurrence of hepatocellular carcinoma(HCC).Methods 71 cases of patients with HCC were grouped into VEGF-positive expression(VP) and VEGF-negative expression(VN).Parameters from the two groups such as classification of cancer cells,tumor capsule,vein tumor thrombus,recurrence rate in 1 year and recurrence rate between 1 and 3 years were compared.Results There were no significant differences between the two groups at classification of cancer cells and recurrence rate between 1 and 3 years(P>0.05).The rate of vein tumor thrombus and recurrence rate in 1 year were higher in group VP than that in group VN(P<0.05).The rate of tumor capsule was lower in group VP than that in group VN(P<0.05).Conclusions Expression of VEGF is correlated with early recurrence of HCC,possibly through the role of micro-metastasis by cancer cells.Expression of VEGF is not significantly correlated with the late recurrence of HCC. References | Related Articles | Metrics

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The diagnostic value of color Doppler ultrasonic on breast malignant tumor LI Hong-xue,LI Hang. 2009, 1 (03):  223-225.  Abstract ( 104 )   Save Objective To explore the features of high frequent ultrasonic(FHUS) image and diagnostic value of color Doppler flow imaging(CDFI) on breast cancer.Methods The features of FHUS image were analyzed retrospectively on 50 cases of malignant breast tumors identified by histopathology.The blood flow parameters of the tumor from patients with different ages,tumor sizes and pathological types were compared.Results Most of the malignant tumors appeared as irregular shape and border,serrated margin,low level and uneven distribution of internal echo.Most of the tumors also showed micro-calcifications,attenuation of rear echo,and the rate of length/width>1.The color Doppler flow imaging grades near the tumors mostly showed abundant blood flow,with the rate of RI≥0.7,and these parameters had close relationship with tumor size.Conclusion The color Doppler flow conditions of breast tumor and the tissues 0.5cm around the tumor have high exactitude for detecting breast cancers.The larger the tumor size,the more abundant of the color Doppler flow.Combination with 2DUS imagines will enhance observably the diagnostic accuracy of breast cancer and then have highly applicable value in clinic. References | Related Articles | Metrics

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The diagnostic value of color Doppler ultrasonic on breast malignant tumor LI Hong-xue,LI Hang. 2009, 1 (03):  223-225.  Abstract ( 70 )   Save Objective To explore the features of high frequent ultrasonic(FHUS) image and diagnostic value of color Doppler flow imaging(CDFI) on breast cancer.Methods The features of FHUS image were analyzed retrospectively on 50 cases of malignant breast tumors identified by histopathology.The blood flow parameters of the tumor from patients with different ages,tumor sizes and pathological types were compared.Results Most of the malignant tumors appeared as irregular shape and border,serrated margin,low level and uneven distribution of internal echo.Most of the tumors also showed micro-calcifications,attenuation of rear echo,and the rate of length/width>1.The color Doppler flow imaging grades near the tumors mostly showed abundant blood flow,with the rate of RI≥0.7,and these parameters had close relationship with tumor size.Conclusion The color Doppler flow conditions of breast tumor and the tissues 0.5cm around the tumor have high exactitude for detecting breast cancers.The larger the tumor size,the more abundant of the color Doppler flow.Combination with 2DUS imagines will enhance observably the diagnostic accuracy of breast cancer and then have highly applicable value in clinic. References | Related Articles | Metrics

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Clinical survey of laparoscopic surgery on colorectal carcinoma 1CHEN Jian-si,2LIU Sheng-wen,QIN Yu-zhou,et al. 2009, 1 (03):  226-228.  Abstract ( 108 )   Save Objective To evaluate the advantages and disadvantages of laparoscopic surgery on colorectal cancer.Methods Clinical records from 22 cases of colorectal carcinoma treated by laparoscopic operation from January 2002 to October 2006 were studied retrospectively.The control group included 40 comparable cases that were treated by conventional surgery in the same period.Results In laparoscopic group,intraoperative blood loss,bowel function restoration time,urinary catheter removal time,out-of-bed activity time and postoperative anodyne consumption were significantly higher than those in conventional group respectively(P<0.05),while the longer mean operation time and higher hospitalization costs were observed in the laparoscopic group.No significant differences were detected between the two groups in tumor size,colorectum specimen length,distal margin and lymph node harvest.The complication rates in the two groups were 9.1% and 32.5% respectively,and the difference was statistically significant(P<0.05).Between the two groups,there were no significant differences in the rates of local recurrence,distant metastasis and mortality after operation.Conclusions Laparoscopic surgery has the same effect as traditional radical operation on colorectal cancer.It is a safe and feasible technique with less surgical trauma and quicker recovery,while with longer mean operation time and higher medical expenses. References | Related Articles | Metrics

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Clinical survey of laparoscopic surgery on colorectal carcinoma 1CHEN Jian-si,2LIU Sheng-wen,QIN Yu-zhou,et al. 2009, 1 (03):  226-228.  Abstract ( 60 )   Save Objective To evaluate the advantages and disadvantages of laparoscopic surgery on colorectal cancer.Methods Clinical records from 22 cases of colorectal carcinoma treated by laparoscopic operation from January 2002 to October 2006 were studied retrospectively.The control group included 40 comparable cases that were treated by conventional surgery in the same period.Results In laparoscopic group,intraoperative blood loss,bowel function restoration time,urinary catheter removal time,out-of-bed activity time and postoperative anodyne consumption were significantly higher than those in conventional group respectively(P<0.05),while the longer mean operation time and higher hospitalization costs were observed in the laparoscopic group.No significant differences were detected between the two groups in tumor size,colorectum specimen length,distal margin and lymph node harvest.The complication rates in the two groups were 9.1% and 32.5% respectively,and the difference was statistically significant(P<0.05).Between the two groups,there were no significant differences in the rates of local recurrence,distant metastasis and mortality after operation.Conclusions Laparoscopic surgery has the same effect as traditional radical operation on colorectal cancer.It is a safe and feasible technique with less surgical trauma and quicker recovery,while with longer mean operation time and higher medical expenses. References | Related Articles | Metrics

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Differential diagnosis of maxillofacial fibrous dysplasia of bone and ossifying fibroma KUANG Hai,CHEN Tao,MENG Ning,et al. 2009, 1 (03):  229-231.  Abstract ( 196 )   Save Objective To study the differentiations between maxillofacial fibrous dysplasia of bone(FD) and ossifying fibroma(OF).Methods Comparative analysis on 44 cases of FD and 66 cases of OF were carried out in the clinical,radiological and pathological manifestations.Results No significant difference was observed in the sex between FD and OF groups.The ages of initiative development and initiative cure,dental lesions,pain on the jaw and face,projection of buccal and/or glossal side of alveolar process in the OF group were higher than that in the FD group,respectively(P<0.05).Mandibulae was more often affected than maxillae in OF group,but there was no significant difference in FD group.The radiological manifestations in OF group presented radiolucent appearance and mixed appearance,while presented ground-glass appearance and mixed appearance in FD group.Conclusion Combinative analysis on clinical,radiological and pathological manifestation is necessary for deferential diagnosis on FD and OF. References | Related Articles | Metrics

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Differential diagnosis of maxillofacial fibrous dysplasia of bone and ossifying fibroma KUANG Hai,CHEN Tao,MENG Ning,et al. 2009, 1 (03):  229-231.  Abstract ( 51 )   Save Objective To study the differentiations between maxillofacial fibrous dysplasia of bone(FD) and ossifying fibroma(OF).Methods Comparative analysis on 44 cases of FD and 66 cases of OF were carried out in the clinical,radiological and pathological manifestations.Results No significant difference was observed in the sex between FD and OF groups.The ages of initiative development and initiative cure,dental lesions,pain on the jaw and face,projection of buccal and/or glossal side of alveolar process in the OF group were higher than that in the FD group,respectively(P<0.05).Mandibulae was more often affected than maxillae in OF group,but there was no significant difference in FD group.The radiological manifestations in OF group presented radiolucent appearance and mixed appearance,while presented ground-glass appearance and mixed appearance in FD group.Conclusion Combinative analysis on clinical,radiological and pathological manifestation is necessary for deferential diagnosis on FD and OF. References | Related Articles | Metrics

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A clinical study on advanced NSCLC treated with the compound of scutellaria barbata kidney-tonifying LU Yuan-zhong1,KONG Qing-zhi2. 2009, 1 (03):  232-234.  Abstract ( 153 )   Save Objective To investigate the clinical effect of scutellaria barbata kidney-tonifying compound on NSCLC stage Ⅲ～Ⅳ.Methods 50 cases in the trial group were treated with the compound of scutellaria barbata youguiyin,and 50 cases in the control group were treated with NP chemotherapy.Results The survival rate of 0.5,1,2,3 years in the trial group were 86%,76%,72% and 70% respectively,while were 92%,80%,68% and 64% respectively in the control group,and the difference between the two groups was not significant(P>0.05).3 years after treatment,the improve rate of life quality in the trial control was 66%,and in the control group was 36%(P<0.01).The late effect rate of tumor body in the trial group was 18%,and in the control group was 60%(P<0.01).The inhibitive rate of marrow in the trial group was 6 %,and in the control group was 48%(P<0.01).The ratio of CD+4/CD+8 in the trial group was 1.46±0.32,and in the control group was1.12±0.26(P<0.05).The activity of NK cell in the trial group was 28.6±6.12,and in the control group was 18.24±4.98(P<0.05).Conclusions Comparing to NP chemotherapy,the treatment of scutellaria barbata youguiyin compound has advantages on high life quality and low toxin side effect. References | Related Articles | Metrics

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A clinical study on advanced NSCLC treated with the compound of scutellaria barbata kidney-tonifying LU Yuan-zhong1,KONG Qing-zhi2. 2009, 1 (03):  232-234.  Abstract ( 95 )   Save Objective To investigate the clinical effect of scutellaria barbata kidney-tonifying compound on NSCLC stage Ⅲ～Ⅳ.Methods 50 cases in the trial group were treated with the compound of scutellaria barbata youguiyin,and 50 cases in the control group were treated with NP chemotherapy.Results The survival rate of 0.5,1,2,3 years in the trial group were 86%,76%,72% and 70% respectively,while were 92%,80%,68% and 64% respectively in the control group,and the difference between the two groups was not significant(P>0.05).3 years after treatment,the improve rate of life quality in the trial control was 66%,and in the control group was 36%(P<0.01).The late effect rate of tumor body in the trial group was 18%,and in the control group was 60%(P<0.01).The inhibitive rate of marrow in the trial group was 6 %,and in the control group was 48%(P<0.01).The ratio of CD+4/CD+8 in the trial group was 1.46±0.32,and in the control group was1.12±0.26(P<0.05).The activity of NK cell in the trial group was 28.6±6.12,and in the control group was 18.24±4.98(P<0.05).Conclusions Comparing to NP chemotherapy,the treatment of scutellaria barbata youguiyin compound has advantages on high life quality and low toxin side effect. References | Related Articles | Metrics

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Diagnostic and therapeutical value of using multi-situs forceps cliping thoracoscopy for malignant pleural effusion DA Chun-he,BIAN Yu-tian,FENG Shuang-cheng,et al. 2009, 1 (03):  235-237.  Abstract ( 90 )   Save Objective To explore the therapeutical effect of medical thoracoscope in diagnosing and treating malignant pleural effusion by using forceps cliping on multi-situs pleura.Methods Multi-situs forceps cliping were performed in 31 patients with malignant pleural effusion.After examination,closed drainage remaining for 3 days and were extubated at the fourth day.The diagnostic rate,curative effect and adverse reaction were compared to the group treated with routine methods.Results In routine methods group,the positive rate,diagnostic rate and final diagnosis rate were 71.0%,35.5% and 19.4% respectively,while all of these three rates were 100% in thoracoscopy group,and the differences between the two groups were significant(P<0.01).Among the 31 patients,21 were complete remission,5 were partial remission,2 were stable and 4 were ineffective.Complete remission rate was 67.7%,and total effective rate was 83.9%.4 cases with pleural effusion recurrence,and the recurrence rate was 19.0%.Complications such as permanence pain,hemorrhage,arrhythmia and pneumothorax did not occur.Conclusions Medical thoracoscope not only has accurate diagnostic value but also has effective treatment effect for malignant pleural effusion.It has superiorities as simple operation,high safety,acceptance by patients,and the examination and therapy are performed simultaneously.Therefore the medical thoracoscopy can be widely applied as a primary treatment for malignant pleural effusion. References | Related Articles | Metrics

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Diagnostic and therapeutical value of using multi-situs forceps cliping thoracoscopy for malignant pleural effusion DA Chun-he,BIAN Yu-tian,FENG Shuang-cheng,et al. 2009, 1 (03):  235-237.  Abstract ( 48 )   Save Objective To explore the therapeutical effect of medical thoracoscope in diagnosing and treating malignant pleural effusion by using forceps cliping on multi-situs pleura.Methods Multi-situs forceps cliping were performed in 31 patients with malignant pleural effusion.After examination,closed drainage remaining for 3 days and were extubated at the fourth day.The diagnostic rate,curative effect and adverse reaction were compared to the group treated with routine methods.Results In routine methods group,the positive rate,diagnostic rate and final diagnosis rate were 71.0%,35.5% and 19.4% respectively,while all of these three rates were 100% in thoracoscopy group,and the differences between the two groups were significant(P<0.01).Among the 31 patients,21 were complete remission,5 were partial remission,2 were stable and 4 were ineffective.Complete remission rate was 67.7%,and total effective rate was 83.9%.4 cases with pleural effusion recurrence,and the recurrence rate was 19.0%.Complications such as permanence pain,hemorrhage,arrhythmia and pneumothorax did not occur.Conclusions Medical thoracoscope not only has accurate diagnostic value but also has effective treatment effect for malignant pleural effusion.It has superiorities as simple operation,high safety,acceptance by patients,and the examination and therapy are performed simultaneously.Therefore the medical thoracoscopy can be widely applied as a primary treatment for malignant pleural effusion. References | Related Articles | Metrics

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Analysis of therapeutic effect and prognosis in nasopharyngeal carcinoma patients with liver metastasis treated by chemotherapy:100 cases report TANG Yi-cong,LU Qiu-xia,ZHAO Li-rong,et al. 2009, 1 (03):  238-241.  Abstract ( 139 )   Save Objective To investigate the clinical features,the effect of chemotherapy and prognostic factors of nasopharyngeal carcinoma(NPC) patients with liver metastasis.Methods A total 100 cases of NPC with liver metastases and received chemotherapy were included in this study.The statistical software SPSS 13.0 was used to analyze retrospectively the clinical data,survival and prognostic factors.Results The median course of chemotherapy was 3.5(1~14),in which 35 cases were more than 4 courses.The cases that used PF programs and non-PF program as the first-line chemotherapy were 71 and 29 respectively,and the effective powers were 57.7% and 42.9% respectively.The difference between PF programs and non-PF program was not significant(P=0.137).In the whole group,the median disease free survival(DFS) was 9 months(0~119),the median survival was 9 months(1~45),the 1-,2-and 3-year survival rates were 32.0%,11.0% and 2.0% respectively.The median survival durations of patients with more than 4 course chemotherapy and patients with less than 4 course chemotherapy were 14 months and 7 months respectively,which was significantly different(P=0.001).Single-factor analysis showed that the prognostic factors of patients with liver metastasis were the course of chemotherapy,complete remission(CR) and radiotherapy on liver metastasis(P=0.002,0.020 and 0.015 respectively).Multi-factor analysis showed that the important prognostic factors were DFS,metastasis before initial treatment,the course of chemotherapy,CR and radiotherapy on liver metastasis.Conclusion Prognosis of NPC with liver metastasis was poor.Multi-systemic chemotherapy should be done to strive CR if patients could tolerate.Palliative radiotherapy that may significantly improve the prognosis can also be tried on some patients. References | Related Articles | Metrics

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Analysis of therapeutic effect and prognosis in nasopharyngeal carcinoma patients with liver metastasis treated by chemotherapy:100 cases report TANG Yi-cong,LU Qiu-xia,ZHAO Li-rong,et al. 2009, 1 (03):  238-241.  Abstract ( 91 )   Save Objective To investigate the clinical features,the effect of chemotherapy and prognostic factors of nasopharyngeal carcinoma(NPC) patients with liver metastasis.Methods A total 100 cases of NPC with liver metastases and received chemotherapy were included in this study.The statistical software SPSS 13.0 was used to analyze retrospectively the clinical data,survival and prognostic factors.Results The median course of chemotherapy was 3.5(1~14),in which 35 cases were more than 4 courses.The cases that used PF programs and non-PF program as the first-line chemotherapy were 71 and 29 respectively,and the effective powers were 57.7% and 42.9% respectively.The difference between PF programs and non-PF program was not significant(P=0.137).In the whole group,the median disease free survival(DFS) was 9 months(0~119),the median survival was 9 months(1~45),the 1-,2-and 3-year survival rates were 32.0%,11.0% and 2.0% respectively.The median survival durations of patients with more than 4 course chemotherapy and patients with less than 4 course chemotherapy were 14 months and 7 months respectively,which was significantly different(P=0.001).Single-factor analysis showed that the prognostic factors of patients with liver metastasis were the course of chemotherapy,complete remission(CR) and radiotherapy on liver metastasis(P=0.002,0.020 and 0.015 respectively).Multi-factor analysis showed that the important prognostic factors were DFS,metastasis before initial treatment,the course of chemotherapy,CR and radiotherapy on liver metastasis.Conclusion Prognosis of NPC with liver metastasis was poor.Multi-systemic chemotherapy should be done to strive CR if patients could tolerate.Palliative radiotherapy that may significantly improve the prognosis can also be tried on some patients. References | Related Articles | Metrics

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Clinical investigation of chemotherapy on gastric bed interstitial tissue during the operation on progressive gastric cancer YUE Jiang-tao,SHI Heng-wei,YUAN Pei-yun,et al. 2009, 1 (03):  242-244.  Abstract ( 85 )   Save Objective To investigate the effect of gastric bed interstitial tissue chemotherapy during the operation on progressive gastric cancer.Methods 164 patients with progression gastric cancer were divided into two groups randomly,84 in therapy group and 80 in control group.The patients' survival rate,no tumor survival rate and relapsing rate of the two groups were observed.Results Between the two groups,the survival rate was not dramatic different in the first year(P>0.05).The survival rate and no tumor survival rate in the second and third years were significantly superior in therapy group than that in control group(P<0.05).The relapse rate in therapy group was also superior than that in control group(P<0.05).Conclusions Chemotherapy on gastric bed interstitial tissue during the operation on progressive gastric cancer can dramatically raise patients' survival rate and survival quality.It can also decrease the relapse rate after operation. References | Related Articles | Metrics

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Clinical investigation of chemotherapy on gastric bed interstitial tissue during the operation on progressive gastric cancer YUE Jiang-tao,SHI Heng-wei,YUAN Pei-yun,et al. 2009, 1 (03):  242-244.  Abstract ( 67 )   Save Objective To investigate the effect of gastric bed interstitial tissue chemotherapy during the operation on progressive gastric cancer.Methods 164 patients with progression gastric cancer were divided into two groups randomly,84 in therapy group and 80 in control group.The patients' survival rate,no tumor survival rate and relapsing rate of the two groups were observed.Results Between the two groups,the survival rate was not dramatic different in the first year(P>0.05).The survival rate and no tumor survival rate in the second and third years were significantly superior in therapy group than that in control group(P<0.05).The relapse rate in therapy group was also superior than that in control group(P<0.05).Conclusions Chemotherapy on gastric bed interstitial tissue during the operation on progressive gastric cancer can dramatically raise patients' survival rate and survival quality.It can also decrease the relapse rate after operation. References | Related Articles | Metrics

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Clinical analysis of treating advanced melanoma with comprehensive immunotherapy MENG Zhi-bin,YU Bin-bin,OU Sheng-qiu,et al. 2009, 1 (03):  245-246.  Abstract ( 179 )   Save Objective To investigate the effect of tumor infiltrating lymphocytes(TIL) stimulated by frozen tumor vaccine on melanoma stage Ⅲ.Methods 42 patients with melanoma stage Ⅲ were divided randomly into treatment group(TIL+IL-2,n=22) and control group(IL-2,n=20).All the patients in both groups were treated for 2 months.The overall survival(OS),relapse-free survival(RFS) and side effect of two groups were compared.Results The OS and RFS showed no significant difference between the treatment group and control group(P>0.05).The RFS and OS from the patients with only one invaded lymph node in treatment group improved significantly than that in control group(all P<0.05).All patients in both groups were well tolerated to the treatments.Conclusions TIL+IL-2 can significantly improve the OS and RFS of patients with melanoma stage Ⅲ.The treatment effect on the patient with only one invaded lymph node is better than that with more than one invaded lymph node. References | Related Articles | Metrics

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Clinical analysis of treating advanced melanoma with comprehensive immunotherapy MENG Zhi-bin,YU Bin-bin,OU Sheng-qiu,et al. 2009, 1 (03):  245-246.  Abstract ( 69 )   Save Objective To investigate the effect of tumor infiltrating lymphocytes(TIL) stimulated by frozen tumor vaccine on melanoma stage Ⅲ.Methods 42 patients with melanoma stage Ⅲ were divided randomly into treatment group(TIL+IL-2,n=22) and control group(IL-2,n=20).All the patients in both groups were treated for 2 months.The overall survival(OS),relapse-free survival(RFS) and side effect of two groups were compared.Results The OS and RFS showed no significant difference between the treatment group and control group(P>0.05).The RFS and OS from the patients with only one invaded lymph node in treatment group improved significantly than that in control group(all P<0.05).All patients in both groups were well tolerated to the treatments.Conclusions TIL+IL-2 can significantly improve the OS and RFS of patients with melanoma stage Ⅲ.The treatment effect on the patient with only one invaded lymph node is better than that with more than one invaded lymph node. References | Related Articles | Metrics