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25 March 2009, Volume 1 Issue 01    Next Issue

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The implications of hOGG1 expression in hepatocellular carcinoma and cirrhosis tissues SU Zhi-xiong,LI Le-qun,PENG Tao,et al. 2009, 1 (01):  3-6.  Abstract ( 243 )   Save Aim:To explore the expression and its clinicopathological implications of human 8-oxoGuanine DNA glycosylase-1 (hOGG1) in hepatocellular carcinoma(HCC) and cirrhotic tissues.Methods:hOGG1 expression was detected by immuneohistochemical EnVision method on liver tissues of non-HCC controls(n =41),cirrhotic(n =51) and HCC tissues(n =99 ).Results: There were three types of hOGG1 positive staining in HCCs:nuclear,cytoplasmic and mixed.The expression levels of hOGG1 in nucleus was significantly higher in HCCs than that in cirrhosis and controls and significantly higher in cirrhosis than that in controls(P<0.01).The expression of hOGG1 was not associated with the clinicopathological factors in HCCs(P>0.05 ).Multiplicity analysis indicated that hOGG1 expression in nucleus was related to ALT(OR = 1.022,P = 0.041),hOGG1 expression in cytoplasm was related to T bili(OR = 1.155,.P = 0.018).Conclusion:During hepatocarcinogenesis,the expression pattern of hOGG1 in liver cell nucleus might reflect the status of oxidative stress in each stage thus has etiology implications. References | Related Articles | Metrics

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The implications of hOGG1 expression in hepatocellular carcinoma and cirrhosis tissues SU Zhi-xiong,LI Le-qun,PENG Tao,et al. 2009, 1 (01):  3-6.  Abstract ( 292 )   Save Aim:To explore the expression and its clinicopathological implications of human 8-oxoGuanine DNA glycosylase-1 (hOGG1) in hepatocellular carcinoma(HCC) and cirrhotic tissues.Methods:hOGG1 expression was detected by immuneohistochemical EnVision method on liver tissues of non-HCC controls(n =41),cirrhotic(n =51) and HCC tissues(n =99 ).Results: There were three types of hOGG1 positive staining in HCCs:nuclear,cytoplasmic and mixed.The expression levels of hOGG1 in nucleus was significantly higher in HCCs than that in cirrhosis and controls and significantly higher in cirrhosis than that in controls(P<0.01).The expression of hOGG1 was not associated with the clinicopathological factors in HCCs(P>0.05 ).Multiplicity analysis indicated that hOGG1 expression in nucleus was related to ALT(OR = 1.022,P = 0.041),hOGG1 expression in cytoplasm was related to T bili(OR = 1.155,.P = 0.018).Conclusion:During hepatocarcinogenesis,the expression pattern of hOGG1 in liver cell nucleus might reflect the status of oxidative stress in each stage thus has etiology implications. References | Related Articles | Metrics

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An experimental study for mAFP gene-modified DC tumor vaccine on transplanted liver cancer XIE Yu-an,KUANG Zhi-peng,LI Yuan-yuan,et al. 2009, 1 (01):  7-9.  Abstract ( 247 )   Save Objective To investigate the inhibitory effect of pAdBM5-mAFP-DC tumor vaccine on the transplanted liver cancer in mice.Methods 40 C57BL/6J mice were randomly divided into group A,B,C,D and E(8 mice in each group).Group A was immunized with mAFP gene-modified DC(pAdBM5-mAFP-DC);Group B was immunized with simple pAdBM5 plasmid; Group C was immunized with recombinant pAdBM5-mAFP plasmid;Group D was immunized with simple DC;Group E was injected with PBS.Each mouse in group A and D was immunized with 5×10~5 cells,in group B and C was immunized with 10.0μg plasmid DNA,in group E was injected with 0.1ml PBS.They were immunized once a week for a consecutive 4 weeks.At the third week,all the mice were transplanted with Hepa1-6 cells.All mice were killed 14 days after Hepa1-6 immunization.Results There was no tumorigenesis could be observed in 3 of 8 mice in group A.The average tumor weight of group A was far more lighter than that in the other groups,with the statistical significance(P<0.05 or 0.01).The tumor-inhibited rate of group A,B,C and D were 68.31%,13.23%,52.1%and 38.54%,respectively.The tumor-inhibited rate for group A was significantly higher than that in the other groups(P<0.05 or 0.01).Conclusion mAFP gene-modified DC tumor vaccine could induce immunoprotection and reduce the risk of tumorigenesis. References | Related Articles | Metrics

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An experimental study for mAFP gene-modified DC tumor vaccine on transplanted liver cancer XIE Yu-an,KUANG Zhi-peng,LI Yuan-yuan,et al. 2009, 1 (01):  7-9.  Abstract ( 228 )   Save Objective To investigate the inhibitory effect of pAdBM5-mAFP-DC tumor vaccine on the transplanted liver cancer in mice.Methods 40 C57BL/6J mice were randomly divided into group A,B,C,D and E(8 mice in each group).Group A was immunized with mAFP gene-modified DC(pAdBM5-mAFP-DC);Group B was immunized with simple pAdBM5 plasmid; Group C was immunized with recombinant pAdBM5-mAFP plasmid;Group D was immunized with simple DC;Group E was injected with PBS.Each mouse in group A and D was immunized with 5×10~5 cells,in group B and C was immunized with 10.0μg plasmid DNA,in group E was injected with 0.1ml PBS.They were immunized once a week for a consecutive 4 weeks.At the third week,all the mice were transplanted with Hepa1-6 cells.All mice were killed 14 days after Hepa1-6 immunization.Results There was no tumorigenesis could be observed in 3 of 8 mice in group A.The average tumor weight of group A was far more lighter than that in the other groups,with the statistical significance(P<0.05 or 0.01).The tumor-inhibited rate of group A,B,C and D were 68.31%,13.23%,52.1%and 38.54%,respectively.The tumor-inhibited rate for group A was significantly higher than that in the other groups(P<0.05 or 0.01).Conclusion mAFP gene-modified DC tumor vaccine could induce immunoprotection and reduce the risk of tumorigenesis. References | Related Articles | Metrics

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The cytolytic effect against liver cancer cells of hIL-2 gene transfected CIK combined with DC KUANG Zhi-peng,WU Ji-ning,LUO Xiao-ling,et al. 2009, 1 (01):  10-13.  Abstract ( 326 )   Save Objective To investigate the cytolytic effect against liver cancer cells of hIL-2 gene transfected cytokine induced killer cells(CIK) combined with dendritic cells(DC).Methods Peripheral blood mononuclear cells(PBMC) were isolated from healthy adult donors by density gradient centrifugation.After 2 hours incubation,CIK was generated from non-adherent cells by anti-CD3McAb,IL-2,IL-1 and IFN-γ.DC was induced from adherent cells by rhIL-4 and rhGM-CSF.Recombinant adenovirus vector pAdBM5-GFP-IL-2 infected CIK at 100 MOI generated IL-2-CIK.DC were pulsed with HepG2 lysate at the ration of 1:3 and generate lysate-DC.The effective cells were lysate-DC-IL2-CIK,lysate-DC-CIK,CIK,DC-CIK and IL2-CIK respectively The target cells were HepG2,K562 and H7404 tumor cell line.The ratio of effective cells and target cells(E/T) were 10:1,20:1 and 40:1 respectively.The cytotoxicity against HepG2,K562 and H7404 was detected with MTT assays. Results There were stronger cytolytic effects against HepG2 for effective cells(Lysate-DC-IL2-CIK) than those effective cells against K562,H7404 and HepG2 in the control group at different E/T ratios.Its cytolytic effects enhanced with the rising of E/T ratio and reached climax when the E/T ratio was 1:40.Conclusions hIL-2 transfected CIK combined with hepatocellular carcinoma antigen pulsed DC has more effective cytolytic effect. References | Related Articles | Metrics

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The cytolytic effect against liver cancer cells of hIL-2 gene transfected CIK combined with DC KUANG Zhi-peng,WU Ji-ning,LUO Xiao-ling,et al. 2009, 1 (01):  10-13.  Abstract ( 245 )   Save Objective To investigate the cytolytic effect against liver cancer cells of hIL-2 gene transfected cytokine induced killer cells(CIK) combined with dendritic cells(DC).Methods Peripheral blood mononuclear cells(PBMC) were isolated from healthy adult donors by density gradient centrifugation.After 2 hours incubation,CIK was generated from non-adherent cells by anti-CD3McAb,IL-2,IL-1 and IFN-γ.DC was induced from adherent cells by rhIL-4 and rhGM-CSF.Recombinant adenovirus vector pAdBM5-GFP-IL-2 infected CIK at 100 MOI generated IL-2-CIK.DC were pulsed with HepG2 lysate at the ration of 1:3 and generate lysate-DC.The effective cells were lysate-DC-IL2-CIK,lysate-DC-CIK,CIK,DC-CIK and IL2-CIK respectively The target cells were HepG2,K562 and H7404 tumor cell line.The ratio of effective cells and target cells(E/T) were 10:1,20:1 and 40:1 respectively.The cytotoxicity against HepG2,K562 and H7404 was detected with MTT assays. Results There were stronger cytolytic effects against HepG2 for effective cells(Lysate-DC-IL2-CIK) than those effective cells against K562,H7404 and HepG2 in the control group at different E/T ratios.Its cytolytic effects enhanced with the rising of E/T ratio and reached climax when the E/T ratio was 1:40.Conclusions hIL-2 transfected CIK combined with hepatocellular carcinoma antigen pulsed DC has more effective cytolytic effect. References | Related Articles | Metrics

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Study on reverse effect of microwave hyperthermia on drug-resistance of MCF-7/ADM cells to ADM LU Yong-kui,HU Xiao-hua,ZHOG Wen-xian,et al. 2009, 1 (01):  14-17.  Abstract ( 379 )   Save Objective Multidrug resistance is one of the main causes of treatment failure of chemotherapy.Reversal agents may be used clinically.This study was to evaluate the reverse effect of microwave hyperthermia on drug-resistance of MCF-7/ ADM to adriamycinum(ADM) cells,and to explore its mechanisms.Methods Breast cell line MCF-7 and its ADM- resistance cell line MCF-7/ADM were treated with ADM and microwave hyperthermia.Reverse effect of microwavehyperthermia on drug-resistance of MCF-7/ADM to ADM was tested by MTT assay.The levels of cellular multidrug resistance related protein(MRP) and P-glycoprotein(P-gp) were detected by Real-Time PCR.The intracellular concentration of ADM was measured by high performance liquid chromatography.Results The resistance index of MCF-7/ADM cells to ADM was 45.5.After microwave hyperthermia, the 50%inhibitory concentration(IC_(50)) of ADM to MCF-7/ADM cells reduced by 2.64 folds from(60.56±2.38)ug/ ml to(22.86±2.76)ug/ml.Protein levels of MRP and P-gp were higher in MCF-7/ADM cells than that in MCF-7 cells,and they were markedly decreased in MCF-7/ADM cells after microwave hyperthermia.The concentration of ADM in MCF-7/ADM cells was significantly increased after microwave hyperthermia.Conclusion Microwave hyperthermia may reverse drug-resistance of MCF-7/ADM cells to ADM through down-regulating MRP and P-gp and increasing intracellular cumulation of ADM. References | Related Articles | Metrics

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Study on reverse effect of microwave hyperthermia on drug-resistance of MCF-7/ADM cells to ADM LU Yong-kui,HU Xiao-hua,ZHOG Wen-xian,et al. 2009, 1 (01):  14-17.  Abstract ( 255 )   Save Objective Multidrug resistance is one of the main causes of treatment failure of chemotherapy.Reversal agents may be used clinically.This study was to evaluate the reverse effect of microwave hyperthermia on drug-resistance of MCF-7/ ADM to adriamycinum(ADM) cells,and to explore its mechanisms.Methods Breast cell line MCF-7 and its ADM- resistance cell line MCF-7/ADM were treated with ADM and microwave hyperthermia.Reverse effect of microwavehyperthermia on drug-resistance of MCF-7/ADM to ADM was tested by MTT assay.The levels of cellular multidrug resistance related protein(MRP) and P-glycoprotein(P-gp) were detected by Real-Time PCR.The intracellular concentration of ADM was measured by high performance liquid chromatography.Results The resistance index of MCF-7/ADM cells to ADM was 45.5.After microwave hyperthermia, the 50%inhibitory concentration(IC_(50)) of ADM to MCF-7/ADM cells reduced by 2.64 folds from(60.56±2.38)ug/ ml to(22.86±2.76)ug/ml.Protein levels of MRP and P-gp were higher in MCF-7/ADM cells than that in MCF-7 cells,and they were markedly decreased in MCF-7/ADM cells after microwave hyperthermia.The concentration of ADM in MCF-7/ADM cells was significantly increased after microwave hyperthermia.Conclusion Microwave hyperthermia may reverse drug-resistance of MCF-7/ADM cells to ADM through down-regulating MRP and P-gp and increasing intracellular cumulation of ADM. References | Related Articles | Metrics

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Serial chemosensitivity test for liver cancer chemotherapy TANG Dong-ping,ZHANG Cui-qing,TANG Kai,et al. 2009, 1 (01):  18-20.  Abstract ( 318 )   Save Objective To investigate the relationship between serial drug sensitivity or resistance to liver cancer cells and peripheral leucocytes,lymphocytes and granulocytes.Methods The sensitivity or resistance of 10 kinds of anticarcinogen for peripheral leucocytes,lymphocytes,granulocytes and the tumor cells from 31 cases of liver cancer were tested by the MTT assay. Results Except HCPT,the inhibition rates of the tested drugs in the cancer cells were apparently higher than that in the leucocytes, lymphocytes and granulocytes.The sensitivity of the top 5 anticarcinogen in cancer cells,leucocytes and lymphocytes were approximately.The correlativity between the inhibition rates of arbitrary two sets cells were all significant(P<0.01). Conclusion It was feasible to detect the sensitivity or resistance of peripheral leucocytes and lymphocytes of liver cancer patients. It could provid practical and valuable reference to optimize the individual treatment in liver cancer chemotherapy. References | Related Articles | Metrics

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Serial chemosensitivity test for liver cancer chemotherapy TANG Dong-ping,ZHANG Cui-qing,TANG Kai,et al. 2009, 1 (01):  18-20.  Abstract ( 248 )   Save Objective To investigate the relationship between serial drug sensitivity or resistance to liver cancer cells and peripheral leucocytes,lymphocytes and granulocytes.Methods The sensitivity or resistance of 10 kinds of anticarcinogen for peripheral leucocytes,lymphocytes,granulocytes and the tumor cells from 31 cases of liver cancer were tested by the MTT assay. Results Except HCPT,the inhibition rates of the tested drugs in the cancer cells were apparently higher than that in the leucocytes, lymphocytes and granulocytes.The sensitivity of the top 5 anticarcinogen in cancer cells,leucocytes and lymphocytes were approximately.The correlativity between the inhibition rates of arbitrary two sets cells were all significant(P<0.01). Conclusion It was feasible to detect the sensitivity or resistance of peripheral leucocytes and lymphocytes of liver cancer patients. It could provid practical and valuable reference to optimize the individual treatment in liver cancer chemotherapy. References | Related Articles | Metrics

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Protein expression of PTEN in endometrial carcinoma tissue and its linical significance JIANG Song-wei,XU Hong,LIANG Xiu-jiu,et al. 2009, 1 (01):  21-23.  Abstract ( 318 )   Save Objective To investigate the expression of tumor suppressor gene PTEN protein in endometrial cancer and its correlation with clinical and pathologic references.Methods The expression of PTEN was detected with immunohistochemical methods in endometrial cancer,endometrial atypical hyperplasia and normal endometrial tissue,and the clinical relevances were analyzed.Results The expression loss rate of PTEN in endometrial cancer was significantly higher than that in endometrial atypical hyperplasia and normal endometrium(P<0.001),and was correlated with the the severity of proliferation and the pathological grade(P<0.05) but not with the depth of myometrial invasion and lymph node metastasis(P>0.05 ).Conclusion The expression loss of PTEN protein has played an important role in the development of endometrial atypical hyperplasia to endometrial cancer. References | Related Articles | Metrics

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Protein expression of PTEN in endometrial carcinoma tissue and its linical significance JIANG Song-wei,XU Hong,LIANG Xiu-jiu,et al. 2009, 1 (01):  21-23.  Abstract ( 249 )   Save Objective To investigate the expression of tumor suppressor gene PTEN protein in endometrial cancer and its correlation with clinical and pathologic references.Methods The expression of PTEN was detected with immunohistochemical methods in endometrial cancer,endometrial atypical hyperplasia and normal endometrial tissue,and the clinical relevances were analyzed.Results The expression loss rate of PTEN in endometrial cancer was significantly higher than that in endometrial atypical hyperplasia and normal endometrium(P<0.001),and was correlated with the the severity of proliferation and the pathological grade(P<0.05) but not with the depth of myometrial invasion and lymph node metastasis(P>0.05 ).Conclusion The expression loss of PTEN protein has played an important role in the development of endometrial atypical hyperplasia to endometrial cancer. References | Related Articles | Metrics

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Correlation between elevated serum DcR3 and TNM staging in gastric carcinoma YANG Mei-songzhu,LIAO Zhi-ling,CHEN Gang,et al. 2009, 1 (01):  24-26.  Abstract ( 308 )   Save Objective To investigate the correlation between serum decoy receptor 3(DcR3) and TNM staging in gastric carcinoma (GC).Methods Serum DcR3 levels were measured by using enzyme linked immunosorbent assay(ELISA) in 34 patients with GC,18 patients with nonmalignant conditions and 28 healthy individuals.Results The levels of serum DcR3 in GC were significantly higher than that in the control group(P<0.01) and the nonmalignant conditions(P<0.01),respectively. Serum DcR3 levels in GC in TNM stageⅢandⅣwere obviously higher than that in stageⅠandⅡ(P<0.01).In addition, patients with both lymph node metastasis and systemic metastasis showed significantly higher serum DcR3 levels compared to that without lymph node metastasis and systemic metastasis(P<0.01,P<0.05).But the levels of serum DcR3 had no correlation with differentiation grades and infiltrate depth(P>0.05,P>0.05).Conclusions Serum DcR3 levels were correlated significantly with TNM stage.This can help to evaluate the pre-operative clinical classification and decide the optimal extent of lymph node and adjacent organs dissection for gastric carcinoma. References | Related Articles | Metrics

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Correlation between elevated serum DcR3 and TNM staging in gastric carcinoma YANG Mei-songzhu,LIAO Zhi-ling,CHEN Gang,et al. 2009, 1 (01):  24-26.  Abstract ( 192 )   Save Objective To investigate the correlation between serum decoy receptor 3(DcR3) and TNM staging in gastric carcinoma (GC).Methods Serum DcR3 levels were measured by using enzyme linked immunosorbent assay(ELISA) in 34 patients with GC,18 patients with nonmalignant conditions and 28 healthy individuals.Results The levels of serum DcR3 in GC were significantly higher than that in the control group(P<0.01) and the nonmalignant conditions(P<0.01),respectively. Serum DcR3 levels in GC in TNM stageⅢandⅣwere obviously higher than that in stageⅠandⅡ(P<0.01).In addition, patients with both lymph node metastasis and systemic metastasis showed significantly higher serum DcR3 levels compared to that without lymph node metastasis and systemic metastasis(P<0.01,P<0.05).But the levels of serum DcR3 had no correlation with differentiation grades and infiltrate depth(P>0.05,P>0.05).Conclusions Serum DcR3 levels were correlated significantly with TNM stage.This can help to evaluate the pre-operative clinical classification and decide the optimal extent of lymph node and adjacent organs dissection for gastric carcinoma. References | Related Articles | Metrics

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Expressions and clinical significance of CD44v6 in breast cancer WEI Chang-yuan,YANG Wei-ping,Zhou Jian-hua,el at. 2009, 1 (01):  27-29.  Abstract ( 305 )   Save Objective To investigate the expression of CD44v6 protein in breast cancer and its clinical significance. Method SP immunohistochemical method was used to assess the expression of CD44v6 protein in 77 mastopathy tissue and the results were correlated with the clinical and data hibtophathologk.Result The expressions of CD44v6 protein in 12 benign cases were all negative.The positive expression rate of CD44v6 protein was 64.6%in the total 65 breast cancer cases.The expression rate in the positive and negative axillary lymph nodes groups were 100%and 25.8%(P<0.05 ) rspectivey;In histopathologic gradeⅠ,ⅡandⅢ,the expression rates were 9.1%,61.8%and 100%,respectively.There were significant differences among the three groups(P<0.001).Logistic multivariate regression analysis showed that the factors affected the expression of CD44v6 were axillary lymph nodes metastasis,tumor size and histopathologic grade.Conclusion CD44v6 protein can be applied as an important indicator in evaluating the malignant transformation,metastases and prognosis of breast cancer. References | Related Articles | Metrics

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Expressions and clinical significance of CD44v6 in breast cancer WEI Chang-yuan,YANG Wei-ping,Zhou Jian-hua,el at. 2009, 1 (01):  27-29.  Abstract ( 174 )   Save Objective To investigate the expression of CD44v6 protein in breast cancer and its clinical significance. Method SP immunohistochemical method was used to assess the expression of CD44v6 protein in 77 mastopathy tissue and the results were correlated with the clinical and data hibtophathologk.Result The expressions of CD44v6 protein in 12 benign cases were all negative.The positive expression rate of CD44v6 protein was 64.6%in the total 65 breast cancer cases.The expression rate in the positive and negative axillary lymph nodes groups were 100%and 25.8%(P<0.05 ) rspectivey;In histopathologic gradeⅠ,ⅡandⅢ,the expression rates were 9.1%,61.8%and 100%,respectively.There were significant differences among the three groups(P<0.001).Logistic multivariate regression analysis showed that the factors affected the expression of CD44v6 were axillary lymph nodes metastasis,tumor size and histopathologic grade.Conclusion CD44v6 protein can be applied as an important indicator in evaluating the malignant transformation,metastases and prognosis of breast cancer. References | Related Articles | Metrics

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Serum concentration of Her-2/neu in patients with advanced breast cancer and its relationship with efficacy of chemotherapy SONG Xiang-qun,ZHOU Jian-hong,YU Qitao.et al. 2009, 1 (01):  30-32.  Abstract ( 263 )   Save Objective To investigate the relationship between efficacy of chemotherapy and serum Her-2/neu concentration in patients with advanced breast cancer,and to analyze its clinical significance.Methods Serum Her-2/neu concentration before and after chemotherapy collected from 66 cases of advanced breast cancer were detected by ELISA and the results were compared with Her-2/neu expression in tissues.The correlation,the efficacy of chemotherapy and time to progress(TTP) were analyzed. Results Serum Her-2/neu concentration was consistent with the expression of Her-2/neu in tumor tissues of patient with advanced breast cancer(P<0.001).The level of Her-2/neu before chemotherapy had no relation to efficacy of chemotherapy (P>0.05).Mean serum Her-2/neu concentration decreased from 33.97ng/ml to 22.11ng/ml after treatment in patient who was effective to chemotherapy,indicating the correlation to response rate of chemotherapy(P<0.001).Conclusion The changes of serum Her-2/neu concentration are significantly associated with efficacy of chemotherapy.Her-2/neu may be used as an indicator in the course of treatment Elevated serum Her-2/neu concentration before chemotherapy was correlated to shorter overall survival. References | Related Articles | Metrics

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Serum concentration of Her-2/neu in patients with advanced breast cancer and its relationship with efficacy of chemotherapy SONG Xiang-qun,ZHOU Jian-hong,YU Qitao.et al. 2009, 1 (01):  30-32.  Abstract ( 180 )   Save Objective To investigate the relationship between efficacy of chemotherapy and serum Her-2/neu concentration in patients with advanced breast cancer,and to analyze its clinical significance.Methods Serum Her-2/neu concentration before and after chemotherapy collected from 66 cases of advanced breast cancer were detected by ELISA and the results were compared with Her-2/neu expression in tissues.The correlation,the efficacy of chemotherapy and time to progress(TTP) were analyzed. Results Serum Her-2/neu concentration was consistent with the expression of Her-2/neu in tumor tissues of patient with advanced breast cancer(P<0.001).The level of Her-2/neu before chemotherapy had no relation to efficacy of chemotherapy (P>0.05).Mean serum Her-2/neu concentration decreased from 33.97ng/ml to 22.11ng/ml after treatment in patient who was effective to chemotherapy,indicating the correlation to response rate of chemotherapy(P<0.001).Conclusion The changes of serum Her-2/neu concentration are significantly associated with efficacy of chemotherapy.Her-2/neu may be used as an indicator in the course of treatment Elevated serum Her-2/neu concentration before chemotherapy was correlated to shorter overall survival. References | Related Articles | Metrics

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Ultrasound-guided mammotome minimally invasive peeling in treatment of benign breast disease ZHANG Ai-ling,ZHANG Rong,ZHANG Yue-huan,et al. 2009, 1 (01):  33-35.  Abstract ( 225 )   Save Objective To evaluate the practical value of ultrasound-guided mammotome minimally invasive peeling on benign breast disease.Methods 856 benign breast masses from 435 patients were treated by ultrasound-guided mammotome minimally invasive peeling.Results All of breast masses were resected safely and completely.Each mass was resected by 18 peelings on average,and the average operation time was 15 minutes.The maximum diameter of the masses measured 1.5±1.0cm,and the lengths of incision were only 3 to 5 mm.No serious complications occurred among the patients.Conclusion Ultrasound-guided mammotome minimally invasive peeling for benign breast disease appears to be a procedure for widespread using because of its simplicity,accuracy,safety and minimally invasiveness. References | Related Articles | Metrics

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Ultrasound-guided mammotome minimally invasive peeling in treatment of benign breast disease ZHANG Ai-ling,ZHANG Rong,ZHANG Yue-huan,et al. 2009, 1 (01):  33-35.  Abstract ( 190 )   Save Objective To evaluate the practical value of ultrasound-guided mammotome minimally invasive peeling on benign breast disease.Methods 856 benign breast masses from 435 patients were treated by ultrasound-guided mammotome minimally invasive peeling.Results All of breast masses were resected safely and completely.Each mass was resected by 18 peelings on average,and the average operation time was 15 minutes.The maximum diameter of the masses measured 1.5±1.0cm,and the lengths of incision were only 3 to 5 mm.No serious complications occurred among the patients.Conclusion Ultrasound-guided mammotome minimally invasive peeling for benign breast disease appears to be a procedure for widespread using because of its simplicity,accuracy,safety and minimally invasiveness. References | Related Articles | Metrics

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Study on the relationship between serum CA_(125) of postoperative chemotherapy and prognosis of epithelial ovarian carcinoma TANG Yong,LI Li,TANG Zhao-qian,et al. 2009, 1 (01):  36-38.  Abstract ( 259 )   Save Objective To investigate the significance of serum CA_(125) value taken after 3 cycle postoperative chemotherapy for evaluating the prognosis of epithelial ovarian carcinoma.Methods Serum CA_(125) after 3 cycle chemotherapy from 94 patients with epithelial ovarian carcinoma treated by operation and 6～8 cycle chemotherapy were detected,during the period from Jan.1, 1998 to Dec.30,2002 in our department.The patients were divided into three groups according to their serum CA_(125) concentration such as normal value(35U/ml),two fold of normal value(70U/ml) and three fold of value(105U/ml),respectively. Accumulated survival rate and average survival time were estimated using Kaplan-Meier estimates and compared with the logrank test where indicated.Results Accumulated survival rate of patients whose 3 cycle postoperative chemotherapy serum CA_(125) were≤35U/ml had no significant difference(P = 0.0693) from that of CA_(125)>35U/ml.There was no significant difference(P = 0.1074) between the group of CA_(125)≤70U/ml and>70U/ml as well.Accumulated survival rate of patients whose 3 cycle postoperative chemotherapy serum CA_(125)≤105 U/ml was significantly higher than those whose CA_(125)>105U/ml(P = 0.0171). Conclusions 105U/ml of CA_(125) after 3 cycle postoperative chemotherapy could be applied to evaluate prognosis of epithelial ovarian carcinoma. References | Related Articles | Metrics

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Study on the relationship between serum CA_(125) of postoperative chemotherapy and prognosis of epithelial ovarian carcinoma TANG Yong,LI Li,TANG Zhao-qian,et al. 2009, 1 (01):  36-38.  Abstract ( 160 )   Save Objective To investigate the significance of serum CA_(125) value taken after 3 cycle postoperative chemotherapy for evaluating the prognosis of epithelial ovarian carcinoma.Methods Serum CA_(125) after 3 cycle chemotherapy from 94 patients with epithelial ovarian carcinoma treated by operation and 6～8 cycle chemotherapy were detected,during the period from Jan.1, 1998 to Dec.30,2002 in our department.The patients were divided into three groups according to their serum CA_(125) concentration such as normal value(35U/ml),two fold of normal value(70U/ml) and three fold of value(105U/ml),respectively. Accumulated survival rate and average survival time were estimated using Kaplan-Meier estimates and compared with the logrank test where indicated.Results Accumulated survival rate of patients whose 3 cycle postoperative chemotherapy serum CA_(125) were≤35U/ml had no significant difference(P = 0.0693) from that of CA_(125)>35U/ml.There was no significant difference(P = 0.1074) between the group of CA_(125)≤70U/ml and>70U/ml as well.Accumulated survival rate of patients whose 3 cycle postoperative chemotherapy serum CA_(125)≤105 U/ml was significantly higher than those whose CA_(125)>105U/ml(P = 0.0171). Conclusions 105U/ml of CA_(125) after 3 cycle postoperative chemotherapy could be applied to evaluate prognosis of epithelial ovarian carcinoma. References | Related Articles | Metrics

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The application of methylene blue subcutaneous injection into areola in sentinel lymph node biopsy for breast cancer QIN Tian,LI Xiao-lin,WU Yong-xiao,et al. 2009, 1 (01):  39-40.  Abstract ( 313 )   Save Objective To inquiry the usefulness of methylene blue subcutaneous injection into areola in sentinel lymph node biopsy for breast cancer.Methods 1%methylene blue 2ml were injected into areola by 4 points before the operation on breast cancer.The lymph node were named as SLN and non-SLN according their locations related to areola.Result The detection rate of sentinel lymph node was 96.4%(54/56).The false negative rate and the false positive rate were 6.1%(2/33) and 0,respectively. Conclusion Subcutaneous injection methylene blue into areola for sentinel lymph node biopsy is helpful for evaluating the lymph node situation of breast cancer. References | Related Articles | Metrics

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The application of methylene blue subcutaneous injection into areola in sentinel lymph node biopsy for breast cancer QIN Tian,LI Xiao-lin,WU Yong-xiao,et al. 2009, 1 (01):  39-40.  Abstract ( 228 )   Save Objective To inquiry the usefulness of methylene blue subcutaneous injection into areola in sentinel lymph node biopsy for breast cancer.Methods 1%methylene blue 2ml were injected into areola by 4 points before the operation on breast cancer.The lymph node were named as SLN and non-SLN according their locations related to areola.Result The detection rate of sentinel lymph node was 96.4%(54/56).The false negative rate and the false positive rate were 6.1%(2/33) and 0,respectively. Conclusion Subcutaneous injection methylene blue into areola for sentinel lymph node biopsy is helpful for evaluating the lymph node situation of breast cancer. References | Related Articles | Metrics

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Application of neuronavigation in neurosurgery operation of intracranial tumors LUO Yu,RUAN Yu-shan,XIAO Shao-wen,et al. 2009, 1 (01):  41-43.  Abstract ( 236 )   Save Objective To explore the application of neuronavigation in microsurgery for intracranial tumors.Methods Fouty-three patients with intracranial tumor were treated by neuronavigational system-assisted microneruosurgery.Results The localization success rate was 100%and the total removal rate was 82.41%(48/58).No severe neurological deficits took place in patients. Conclusion The Stealth Station neuronavigational system is reliable and accurate for neurosurgical operation.Thus it is helpful to facilitate total resection of intracranial tumors with less operative complications. References | Related Articles | Metrics

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Application of neuronavigation in neurosurgery operation of intracranial tumors LUO Yu,RUAN Yu-shan,XIAO Shao-wen,et al. 2009, 1 (01):  41-43.  Abstract ( 180 )   Save Objective To explore the application of neuronavigation in microsurgery for intracranial tumors.Methods Fouty-three patients with intracranial tumor were treated by neuronavigational system-assisted microneruosurgery.Results The localization success rate was 100%and the total removal rate was 82.41%(48/58).No severe neurological deficits took place in patients. Conclusion The Stealth Station neuronavigational system is reliable and accurate for neurosurgical operation.Thus it is helpful to facilitate total resection of intracranial tumors with less operative complications. References | Related Articles | Metrics

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The efficacy of three-dimensional conformal radiotherapy plus transcatheter arterial chemoembolization for treatment of primary liver cancer LIANG Xia,ZHU Xia-dong,LIANG Shi-xiong,et al. 2009, 1 (01):  44-46.  Abstract ( 270 )   Save Objective To evaluate the therapeutic effects of three-dimensional conformal radiation(3DCRT) combined with transcatheter arterial chemoembolization(TACE) on the unresectable primary liver cancer(PLC).Methods From April 1999 to July 2007,a total of 83 patients who could not receive surgical therapy were treated with TACE plus 3DCRT.Fractionated radiotherapy dose was 2～8Gy(median 4.6Gy),irradiation times were 6～30(median 11),3～5 times/week,tumor dose was 33.6～70Gy(median 52Gy).TACE was performed by using lipiodol,cisplatin,perarubicin,mitomycin,gemcitabine hydrochloride, hydroxycamptothecin and gelatin sponge cubes.Results The short-term efficacy rate was 42.2%.The 1-,2- and 3- year survival rates were 65.9%,32.5%and 25.5%,respectively.The liver function tests showed abnomality in one patient, upper gastrointestinal hemorrhage in three patients,Ⅰ～Ⅱlevel inhibitory effect on marrow function in 16 patients and fever in one patient Conclusion The 3DCRT is effective,feasible and can be applied as one of the first-line means for treating unresectable PLC. References | Related Articles | Metrics

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The efficacy of three-dimensional conformal radiotherapy plus transcatheter arterial chemoembolization for treatment of primary liver cancer LIANG Xia,ZHU Xia-dong,LIANG Shi-xiong,et al. 2009, 1 (01):  44-46.  Abstract ( 174 )   Save Objective To evaluate the therapeutic effects of three-dimensional conformal radiation(3DCRT) combined with transcatheter arterial chemoembolization(TACE) on the unresectable primary liver cancer(PLC).Methods From April 1999 to July 2007,a total of 83 patients who could not receive surgical therapy were treated with TACE plus 3DCRT.Fractionated radiotherapy dose was 2～8Gy(median 4.6Gy),irradiation times were 6～30(median 11),3～5 times/week,tumor dose was 33.6～70Gy(median 52Gy).TACE was performed by using lipiodol,cisplatin,perarubicin,mitomycin,gemcitabine hydrochloride, hydroxycamptothecin and gelatin sponge cubes.Results The short-term efficacy rate was 42.2%.The 1-,2- and 3- year survival rates were 65.9%,32.5%and 25.5%,respectively.The liver function tests showed abnomality in one patient, upper gastrointestinal hemorrhage in three patients,Ⅰ～Ⅱlevel inhibitory effect on marrow function in 16 patients and fever in one patient Conclusion The 3DCRT is effective,feasible and can be applied as one of the first-line means for treating unresectable PLC. References | Related Articles | Metrics

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Analysis on prognosis and response to chemotherapy of triplenegative breast cancer CAO Yi-lin,XU Li-rong,QU Yuan-jiao,et al. 2009, 1 (01):  47-48.  Abstract ( 336 )   Save Objective To evaluate the response to chemotherapy and the prognosis of triple-negative breast cancer(TNBC)by comparing to the non-TNBC.Methods 147 advanced breast carcinoma patients were defined negative expresions of estrogenr eceptor(ER),progesterone receptor(PR) and human epidermal growth factor rector 2(Her-2 ) by immunohistochemistry. Paclitaxel or Docetaxel was administered intravenously at a dose of 135mg/m~2(or 75 mg/m~2)on d1,Cisplatin 30mg/m~2 from d1 to d3.A treatment cycle was 21 days.After 2 cycles,the pathologic parameters,the treatment efficacy and the prognosis were compared between the patients of TNBC and non-TNBC.Results 20 of 147 patients(13.6%) were TNBC.Patients of TNBC had higher overall response rate compared with non-TNBC(P = 0.048 ).The median progression free survial time in the patients of TNBC and non-TNBC were 5.5 months and 6.5 months respectively.There were significant difference between the two groups at primary tumor size(P =0.042).Conclusion Paclitaxel or Docetaxel combined with Cisplatin chemotherapy regimen was sensitive in TNBC,but the median progression free survial time was worse in this group. References | Related Articles | Metrics

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Analysis on prognosis and response to chemotherapy of triplenegative breast cancer CAO Yi-lin,XU Li-rong,QU Yuan-jiao,et al. 2009, 1 (01):  47-48.  Abstract ( 245 )   Save Objective To evaluate the response to chemotherapy and the prognosis of triple-negative breast cancer(TNBC)by comparing to the non-TNBC.Methods 147 advanced breast carcinoma patients were defined negative expresions of estrogenr eceptor(ER),progesterone receptor(PR) and human epidermal growth factor rector 2(Her-2 ) by immunohistochemistry. Paclitaxel or Docetaxel was administered intravenously at a dose of 135mg/m~2(or 75 mg/m~2)on d1,Cisplatin 30mg/m~2 from d1 to d3.A treatment cycle was 21 days.After 2 cycles,the pathologic parameters,the treatment efficacy and the prognosis were compared between the patients of TNBC and non-TNBC.Results 20 of 147 patients(13.6%) were TNBC.Patients of TNBC had higher overall response rate compared with non-TNBC(P = 0.048 ).The median progression free survial time in the patients of TNBC and non-TNBC were 5.5 months and 6.5 months respectively.There were significant difference between the two groups at primary tumor size(P =0.042).Conclusion Paclitaxel or Docetaxel combined with Cisplatin chemotherapy regimen was sensitive in TNBC,but the median progression free survial time was worse in this group. References | Related Articles | Metrics

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Clinical Analysis of Interventional Procedure in Primary Hepatic Cancer ZHANG Yang,MA Yi-long,KANG Ping,et al. 2009, 1 (01):  49-52.  Abstract ( 246 )   Save Objective By comparing interventional treatments TACE,TACE + PEI and PEI for primary liver cancer(PLC) and comprehensivly analysing major factors which influenced prognosis,to provide a theory-based evidence for the formulation of treatment plan,the effects of interventional treatment and the estimation of prognosis.Methods 98 PLC patients treated with TACE,PEI or TACE + PEI were studied retrospectively.Cox regression analysis was used for the impact on treatment and prognostic of liver cancer-related factors.Results Of the three groups,TACE + PEI group obtained the best curative effect,the highest survival rate and the longest middle life span.The best achievement on reducing AFP value and the improvement of liver functional also happened in TACE + PEI group.Cox regression simplex factor analysis showed that the significantly influential factors were carcinoma embolus,tumour diminution rate,TACE + PEI treatment and AFP value of post-treatment Multiplicity factor analysis showed that carcinoma embolus and TACE + PEI treatment were the factors make sense to prognosis.Conclusion TACE + PEI treatment has notably curative effect With TACE + PEI treatment,the decrease of AFP value after treatment and the improvement of liver function on paients also obviously better than teated with either TACE or PEI.Besides,this treatment can prolong sufferers life span notably.The formation of carcinoma embolus in portal vein is the the most important risk factor to impact prognosis of patients suffered PLC with interventional treatment.Paients without carcinoma embolus and given TACE + PEI treatment have longer life span. References | Related Articles | Metrics

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Clinical Analysis of Interventional Procedure in Primary Hepatic Cancer ZHANG Yang,MA Yi-long,KANG Ping,et al. 2009, 1 (01):  49-52.  Abstract ( 173 )   Save Objective By comparing interventional treatments TACE,TACE + PEI and PEI for primary liver cancer(PLC) and comprehensivly analysing major factors which influenced prognosis,to provide a theory-based evidence for the formulation of treatment plan,the effects of interventional treatment and the estimation of prognosis.Methods 98 PLC patients treated with TACE,PEI or TACE + PEI were studied retrospectively.Cox regression analysis was used for the impact on treatment and prognostic of liver cancer-related factors.Results Of the three groups,TACE + PEI group obtained the best curative effect,the highest survival rate and the longest middle life span.The best achievement on reducing AFP value and the improvement of liver functional also happened in TACE + PEI group.Cox regression simplex factor analysis showed that the significantly influential factors were carcinoma embolus,tumour diminution rate,TACE + PEI treatment and AFP value of post-treatment Multiplicity factor analysis showed that carcinoma embolus and TACE + PEI treatment were the factors make sense to prognosis.Conclusion TACE + PEI treatment has notably curative effect With TACE + PEI treatment,the decrease of AFP value after treatment and the improvement of liver function on paients also obviously better than teated with either TACE or PEI.Besides,this treatment can prolong sufferers life span notably.The formation of carcinoma embolus in portal vein is the the most important risk factor to impact prognosis of patients suffered PLC with interventional treatment.Paients without carcinoma embolus and given TACE + PEI treatment have longer life span. References | Related Articles | Metrics

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The application of Orem's self-care theory on fatigue of breast cancer patients during chemotherapy JIANG Jin-fang,LAO Yong-cong,CHEN Mei-zhong,et al. 2009, 1 (01):  57-58.  Abstract ( 377 )   Save Objective To investigate the effect of the nursing model of Orem' s self-care theory in reducing fatigue of breast cancer patients during chemotherapy.Methods 60 breast cancer patients duringer chemotherapy were randomly divided into the test group that adopted Orem' s self-care theory of nursing and the control group that adopted the conventional nursing method.The self-care theory was applied in the test group to evaluate their self-care ability,and self-care nursing intervention wasestablished to help,guide,educate and train thems.Piper fatigue scale(PFS) was used to assess the fatigue level at 2 days before and 7 days after chemotherapy.Results Patient' s fatigue was reduced significantly in the test group after chemotherapy (P<0.01).Conclusion The nursing model of Orem' s self-care theory can effectively reduce fatigue of breast cancer patients during chemotherapy. References | Related Articles | Metrics

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The application of Orem's self-care theory on fatigue of breast cancer patients during chemotherapy JIANG Jin-fang,LAO Yong-cong,CHEN Mei-zhong,et al. 2009, 1 (01):  57-58.  Abstract ( 301 )   Save Objective To investigate the effect of the nursing model of Orem' s self-care theory in reducing fatigue of breast cancer patients during chemotherapy.Methods 60 breast cancer patients duringer chemotherapy were randomly divided into the test group that adopted Orem' s self-care theory of nursing and the control group that adopted the conventional nursing method.The self-care theory was applied in the test group to evaluate their self-care ability,and self-care nursing intervention wasestablished to help,guide,educate and train thems.Piper fatigue scale(PFS) was used to assess the fatigue level at 2 days before and 7 days after chemotherapy.Results Patient' s fatigue was reduced significantly in the test group after chemotherapy (P<0.01).Conclusion The nursing model of Orem' s self-care theory can effectively reduce fatigue of breast cancer patients during chemotherapy. References | Related Articles | Metrics